Methods for conducting bioassays using embryos and larvae of Pacific herring, Clupea pallasi.
نویسندگان
چکیده
The rapid decrease of several stocks of Pacific herring, Clupea pallasi, in Puget Sound, Washington, has led to concerns about the effects of industrial and nonpoint source contamination on the embryo and larval stages of this and related forage fish species. To address these concerns, the state of Washington and several industries have funded efforts to develop embryo and larval bioassay protocols that can be used by commercial laboratories for routine effluent testing. This article presents the results of research to develop herring embryo and larval bioassay protocols. Factors evaluated during protocol development included temperature, salinity, dissolved oxygen (DO), light intensity, photoperiod, larval feeding regimes, use of brine and artificial sea salts, gonad sources, collection methods, and egg quality.
منابع مشابه
Impacts of suspended sediments on fertilization, embryonic development, and early larval life stages of the pacific herring, Clupea pallasi.
Pacific herring reproduce in the San Francisco Bay estuary during times of the year when suspended sediment loads are highest due to freshwater input, yet little is known about the effects of sediment on herring early life stages. During the first 2 h after eggs contacted water, embryos were adhesive and susceptible to having sediment particles attach permanently to the chorion. Treatment with ...
متن کاملThe onset of yolk-sac edema in Pacific herring (Clupea pallasi) due to environmental stressors present during an oil spill in an estuarine environment
OPEN ACCESS This open-access article is distributed under the terms of the Creative Commons Attribution Non-Commercial License (CC BY-NC) (http:// creativecommons.org/licenses/by-nc/4.0/), which permits reuse, distribution and reproduction of the article, provided that the original work is properly cited and the reuse is restricted to noncommercial purposes. For commercial reuse, contact reprin...
متن کاملAssociation of plasma IgM with body size, histopathologic changes, and plasma chemistries in adult Pacific herring Clupea pallasi.
Pacific herring Clupea pallasi immunoglobulin is an IgM-like molecule comprised of heavy and light chains with molecular weights of 79 and 25 to 27 kD, respectively. Purified immunoglobulin was used to generate highly specific polyclonal antibodies for development of a sandwich ELISA. The ELISA was used to quantify total plasma IgM in 602 Pacific herring captured in Prince William Sound and Sit...
متن کاملAppendix 1I: Spawning Habitat Selection by Herring in Kamishak Bay, AK Statistical Analysis A total of 488,653 km of shoreline in Kamishak Bay, AK was surveyed for Pacific herring (Clupea pallasi) spawning events from fixed-wing aircraft in years
Statistical Analysis A total of 488,653 km of shoreline in Kamishak Bay, AK was surveyed for Pacific herring (Clupea pallasi) spawning events from fixed-wing aircraft in years 1978 – 2001, with the exception of 1982 and 1997. Every survey followed the same route. Although multiple surveys occurred in each year, the survey frequency was largely a function of weather, perceived size of the spawni...
متن کاملPhotoenhanced toxicity of aqueous phase and chemically dispersed weathered Alaska North Slope crude oil to Pacific herring eggs and larvae.
The photoenhanced toxicity of weathered Alaska North Slope crude oil (ANS) was investigated in the eggs and larvae of Pacific herring (Clupea pallasi) with and without the chemical dispersant Corexit 9527. Oil alone was acutely toxic to larvae at aqueous concentrations below 50 microg/L total polycyclic aromatic hydrocarbons (tPAH), and median lethal (LC50s) and effective concentrations (EC50s)...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Archives of environmental contamination and toxicology
دوره 60 2 شماره
صفحات -
تاریخ انتشار 2011